Journal: Scientific Reports

Article Title: Dietary polyamines promote intestinal adaptation in an experimental model of short bowel syndrome

doi: 10.1038/s41598-024-55258-4

Figure Lengend Snippet: Polyamines enhance mucosal defense factors in rats with massive intestinal resection. (A-B) Fecal and serum secretory IgA was measured using ELISA. n = 5–7/group. (C) IgA in ileum tissue was assessed using western blotting. n = 4–6/group. (D) Fecal mucin was measured using a fluorometric assay. n = 4–6/group. (E) Representative images of ileal villus showing immunostaining by Muc2 (original magnification, ×400; scale bars = 200 μm). Left graphs show the number of goblet cells per unit villous area and the size of goblet cell secretion granule. (F) The expression of Claudin-3 in the ileum tissue was measured by western blot analysis. Representative images of ileal villus showing immunostaining by Claudin-3 (original magnification, ×200; scale bars = 100 μm). (G) Serum DAO was measured by ELISA. n = 5–7/group. (H) Serum GLP-2 was measured by ELISA. n = 5–7/group. (I) Fecal short-chain fatty acid (SCFA) content was measured using high-performance liquid chromatography. n = 3–6/group. Data are presented as mean ± SD. Results of one-way ANOVA are represented as follows: * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001.

Article Snippet: Nonspecific antibody binding was blocked using 15% goat serum for 30 min. For staining of Ki-67, Muc2, and Claudin-3, the slides were then incubated with anti-Ki67 antibody (ab16667; Abcam, Cambridge, UK), anti-Muc2 rabbit monoclonal antibody (M01212-1; Boster Biological Technology, Pleasanton, CA, USA), and Claudin-3 polyclonal antibody (#PA5-32353; Invitrogen, Waltham, MA, USA) at 4°C overnight, respectively.

Techniques: Enzyme-linked Immunosorbent Assay, Western Blot, Immunostaining, Expressing, High Performance Liquid Chromatography

Journal: Investigative Ophthalmology & Visual Science

Article Title: Ectoine Enhances Mucin Production Via Restoring IL-13/IFN-γ Balance in a Murine Dry Eye Model

doi: 10.1167/iovs.65.6.39

Figure Lengend Snippet: Antibodies Used in This Study

Article Snippet: MUC2 , CUSABIO , PA207869 , Rabbit , 1:3000.

Techniques:

Journal: Investigative Ophthalmology & Visual Science

Article Title: Ectoine Enhances Mucin Production Via Restoring IL-13/IFN-γ Balance in a Murine Dry Eye Model

doi: 10.1167/iovs.65.6.39

Figure Lengend Snippet: Mouse TaqMan Gene Expression Assays Used in This Study

Article Snippet: MUC2 , CUSABIO , PA207869 , Rabbit , 1:3000.

Techniques: Gene Expression

Journal: Investigative Ophthalmology & Visual Science

Article Title: Ectoine Enhances Mucin Production Via Restoring IL-13/IFN-γ Balance in a Murine Dry Eye Model

doi: 10.1167/iovs.65.6.39

Figure Lengend Snippet: Ectoine enhances production of secreted mucins in DS mice. ( A , C ) Representative images of IF staining showing the decreased production of MUC5AC ( A ) and MUC2 ( C ) in DS mice (DS+PBS) was recovered by ectoine eye drops (DS+Ect); Scale bars = 50 µm. The stitched images were made because the cornea and conjunctiva in some eyeball sections were too far apart to be captured on the same image. ( B , D ) IF intensity quantified the alternation of MUC5AC and MUC2 in 3 groups; each circle represents one eye. Data are shown as Mean ± SD, n = 6; the P values are shown in the graph as compared with the DS+PBS group.

Article Snippet: MUC2 , CUSABIO , PA207869 , Rabbit , 1:3000.

Techniques: Staining, Eye Drops

Journal: Investigative Ophthalmology & Visual Science

Article Title: Ectoine Enhances Mucin Production Via Restoring IL-13/IFN-γ Balance in a Murine Dry Eye Model

doi: 10.1167/iovs.65.6.39

Figure Lengend Snippet: Gene expression of MUC5AC, MUC2, MUC1, MUC4, MUC16 , and MUC15 by conjunctiva and cornea. ( A ) Gene expression of all 6 mucins in normal mice were detected at different mRNA levels based on the threshold cycle (Ct) value in real-time PCR with house-keeping gene GAPDH as an internal control. ( B ) The mRNA levels of all six mucins in conjunctiva of the three groups of mice. ( C ) The mRNA expression of MUC1, MUC4, and MUC16 by corneas in the three groups of mice. Data are shown as Mean ± SD, n = 6; the P values are shown in the graph as compared with the DS+PBS group.

Article Snippet: MUC2 , CUSABIO , PA207869 , Rabbit , 1:3000.

Techniques: Gene Expression, Real-time Polymerase Chain Reaction, Control, Expressing

Journal: International Journal of Molecular Medicine

Article Title: Intestinal changes in permeability, tight junction and mucin synthesis in a mouse model of Alzheimer's disease

doi: 10.3892/ijmm.2023.5316

Figure Lengend Snippet: Monoclonal primary antibodies in western blotting.

Article Snippet: Sections were immunostained with a rabbit anti-MUC2 antibody (1:100; red; cat. no. #DF8390; Affinity Biosciences) and incubated at 4°C overnight.

Techniques: Western Blot

Journal: International Journal of Molecular Medicine

Article Title: Intestinal changes in permeability, tight junction and mucin synthesis in a mouse model of Alzheimer's disease

doi: 10.3892/ijmm.2023.5316

Figure Lengend Snippet: Variation in number of GCs and MUC2 expression in intestinal mucosa. (Aa-h) Alcian blue-Periodic acid Schiff staining showed GCs and (Ba-h) immunohistochemistry showed MUC2 in the ileum and proximal colon of WT and dTg mice at 6 and 12 months. (C) Number of GCs, as determined by histochemical staining of the ileum and proximal colon. (D) MUC2 expression area in the ileum and proximal colon was determined by immunohistochemistry (n=6/group). Scale bar, 50 µ m. (E) Protein levels of MUC2 in extracts of intestinal tissue were detected by western blotting. (F) Quantification of the western blot results (n=4/group). * P<0.05, ** P<0.01. GC, goblet cell; WT, wild-type; dTg, double transgene; MUC2, mucin 2; WGA, wheat germ agglutin.

Article Snippet: Sections were immunostained with a rabbit anti-MUC2 antibody (1:100; red; cat. no. #DF8390; Affinity Biosciences) and incubated at 4°C overnight.

Techniques: Expressing, Staining, Immunohistochemistry, Western Blot

Journal: International Journal of Molecular Medicine

Article Title: Intestinal changes in permeability, tight junction and mucin synthesis in a mouse model of Alzheimer's disease

doi: 10.3892/ijmm.2023.5316

Figure Lengend Snippet: Variation in MUC2 and WGA in intestinal mucosa. (Aa-p) IF showed MUC2 and WGA in ileum of WT and dTg mice at 6 and 12 months. (B) MUC2- and (C) WGA-positive area was determined by IF in ileum (n=6/group). IF showed MUC2 and WGA in (Da-p) proximal colon of WT and dTg mice at 6 and 12 months. (E) MUC2- and (F) WGA-positive area was determined by IF in proximal colon (n=6/group). * P<0.05, ** P<0.01. WGA, wheat germ agglutinin; IF, immunofluorescence; MUC2, mucin 2; WT, wild-type; dTg, double transgene.

Article Snippet: Sections were immunostained with a rabbit anti-MUC2 antibody (1:100; red; cat. no. #DF8390; Affinity Biosciences) and incubated at 4°C overnight.

Techniques: Immunofluorescence